Isolation, characterization, and utilization of T lymphocyte clones

Publisher: Academic Press in New York

Written in English
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Published: Pages: 549 Downloads: 634
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  • T cells.,
  • Clone cells.

Edition Notes

Includes bibliographies and index.

Statementedited by C. Garrison Fathman, Frank W. Fitch ; contributors, M.E. Andrew ... [et al.].
ContributionsFathman, C. Garrison., Fitch, Frank Wesley, 1929-, Andrew, M. E.
LC ClassificationsQR185.8.L9 I86 1982
The Physical Object
Paginationxxviii, 549 p. :
Number of Pages549
ID Numbers
Open LibraryOL3512190M
ISBN 100122499204
LC Control Number82072508

Isolation of lymphocytes from pancreas, LN, spleen and thymus Method 1. Perform cardiac perfusion (40 ml PBS/mouse) and harvest pancreata, PLN, CLN, thymi & spleen. Make sure to pick pancreas clean of all LN. NB. Process all tissues in the same manner (cutting with scissors into 1 mm pieces and placing in digestion buffer etc). 2. We describe a method for generation of homogeneous cell populations that each arise from clonal expansion of cells at a discrete stage of differentiation within a single lineage. We have used this to produce continuously propagatable lymphocyte clones. Each clone represents a cell at a progressive stage of thymus-dependent cellular differentiation. Isolation and Characterization of Interleukin‐1 From Bovine Polymorphonuclear Leukocytes The DlO G4. 1 murine T-helper cell clone was we examined the numbers and percent of T lymphocyte. Goldsmith MA, Weiss A. Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor. Proc. Natl. Acad. Sci. USA , PubMed: Lund T, et al. Herpesvirus saimiri Tip membrane protein markedly increases p56lck activity in T cells. J. Virol. ,

@article{osti_, title = {Isolation and characterization of full-length cDNA clones coding for cholinesterase from fetal human tissues}, author = {Prody, C A and Zevin-Sonkin, D and Gnatt, A and Goldberg, O and Soreq, H}, abstractNote = {To study the primary structure and regulation of human cholinesterases, oligodeoxynucleotide probes were prepared according to a consensus peptide. Cell Isolation Cell Characterization To assess purity or characterize lymphocytes, choose from our wide selection of antibodies optimized for flow cytometry, microscopy and other applications.   Weiss A, et al. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J. Immunol. , PubMed: Gillis S, Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V.   Current Protocols in Immunology is a comprehensive source for protocols and overviews in the field of immunology including, in vivo and in vitro assays to study lymphocyte cells, the isolation and differentiation of stem and progenitor cells, flow cytometry and cell sorting, and animal models of infections disease.

Here, we report on the isolation of a full length cDNA clone, encoding the WC1 Ag, from a COS cell cDNA expression library prepared from a bovine gamma delta T cell line. The protein encoded by the pWC1 cDNA clone was reactive with the bovine mAb CC15 and IL.A29, and with T   By sequence analysis of cDNA clones, Sandrin et al. () demonstrated that the mouse lymphocyte surface antigen Ly9 is a novel member of a subgroup of the immunoglobulin superfamily, together with the antigens CD2 (), BCM1 (CD48; ), and LFA3 (CD58; ). Kingsmore et al. () noted that, though the function of Ly9 was unknown, other subgroup . Isolation and characterization of virus-specific T cells from two donors resulted in the identification of two CD8 + T cell epitopes on the HTNV N protein, which were restricted by either HLA A1 or B These CTL lines included both HTNV-specific (HLA Brestricted) . @article{osti_, title = {Isolation and characterization of cDNA clones for carrot extensin and a proline-rich kDa protein}, author = {Chen, J and Varner, J E}, abstractNote = {Extensins are hydroxyproline-rich glycoproteins associated with most dicotyledonous plant cell walls. To isolate cDNA clones encoding extensin, the authors started by isolating poly(A) RNA from carrot root.

Isolation, characterization, and utilization of T lymphocyte clones Download PDF EPUB FB2

Isolation, Characterization, and Utilization of T Lymphocyte Clones is a summary of information regarding T lymphocyte clones, including their Edition: 1. Isolation, Characterization, and Utilization of T Lymphocyte Clones is a summary of information regarding T lymphocyte clones, including their usefulness.

The book then looks into the analysis of T cell clones and hybridomas using techniques of somatic cell genetics. The clonal analysis by limiting dilution, the characteristics of murine T.

Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (K), or click on a page image below to browse page by by: Publisher List Price: $ Savings: $ Isolation Characterization, and Utilization of T Lymphocyte Clones.

Subject: Allied Health & Medical -> Medical -> Immunology. Isolation, Characterization, and Utilization of T Lymphocyte Clones is a summary of information regarding T lymphocyte clones, including their usefulness. Organized into nine parts, the book begins with discussions on the soluble factors that can influence the growth of cloned T cells and the utilization of T cell hybridomas for analysis of T cell functions, emphasizing the biochemical and.

The purified population of the lamprey lymphocyte-like cells was used as a source for RNA isolation, production and utilization of T lymphocyte clones book a cDNA library, and sequencing of randomly selected cDNA clones.

In the collection of 8, sequences remaining after the exclusion of poor-quality sequences from the initial number of 9, there were 1, that were represented.

The demonstra tion of acid characterization acetate esterase activity in human lymphocytes: Usefulness as a T-cell marker. Cell. Immunol. 3 5, Kulenkampff, J., Janossy, G., and G r e a v e s, M. Acid esterase in human lymphoid cells and leukaemic blasts: A marker for T lymphocytes.

Haematol. 36, 2 3 1 - 2 4 0. This chapter presents T cell cloning and hybrid production with the basic approaches and methods required for performing karyotypic analysis on cell l.


Nabholz M () The somatic cell genetic analysis of cytolytic T lymphocyte functions. In Fathman CG, Fitch FW (eds) Isolation, Characterization, and Utilization of T Lymphocyte Clones. Academic Press, New York, p Google Scholar. Isolation, characterization and.

utilization of T lymphocyte clones. Academic Press, New York. Cytolytic T lymphocytes (CTLs) are important effectors in the recognition of viruses.

Krammer PH, Dy M, Hültner L, Isakson P, Kees U, Lohmann-Mattes ML, Marcucci F, Michnay A, Puré E, Schimpl A, Staher F, Vitetta E, Waller M: Production of lymphokines by murine T cells grown in limiting dilution and long-term cultures.

In: Isolation, Characterization, and Utilization of T Lymphocyte clones. Fathman G, Fitch F, eds. Academic Press. We have generated continuously propagatable T lymphocyte clones to study antigen-specific T cell functions. All Ly-2+ clones mediate suppressive activity and secrete a characteristic pattern of polypeptides that differs from Ly T cell clones.

Cells of one clone, 23/4, specifically bind glycophorin from sheep erythrocytes (SRBC). Nabholz, M. The somatic cell genetic analysis of cytolytic T- lymphocyte functions. “Isolation, Characterization and Utilization of T Lymphocyte Clones.” G. Fathman and F.W. Fitch, eds., Academic Press, in press.

Google Scholar. In Isolation, Characterization and Utilization of T Lymphocyte Clones, eds. C.G. Fathman and F.W. Fitch, pp. – New York: Academic Press. Discover the best Cloning books and audiobooks.

Learn from Cloning experts like Elsevier Books Reference and I.D. Anton, PhD. Read Cloning books like Principles of Cloning and Somatic Embryogenesis for Micropropagation of Coconut (Cocos nucifera L.) with a free trial.

Isolation and characterization of hematopoietic stem cells in teleost fish Isao Kobayashi1), Fumihiko Katakura 2), and *Tadaaki Moritomo 1)Faculty of Natural System, Institute of Science and Engineering, Kanazawa University Kakumamachi, Kanazawa, IshikawaJapan.

We are looking to extract CD4+ T cells from diseased mouse skin for lymphocyte analysis. So far the protocols we have tried either yield only ~ cells from a 1 cm sq of skin (healthy c57Bl/6.

With the development of in vitro cloning techniques, it is now possible to examine directly the T lymphocytes which produce this and other lymphokines (4–9). Here we describe the phenotypes, antigen stimulation requirements and characteristics of lymphokine production by 72 clones generated against H-2, Mls, H-Y or Moloney leukemia virus.

A clone-specific monoclonal antibody that inhibits cytolysis of a cytolytic T cell clone. J Exp Med. Mar 1; (3)– [PMC free article] Moldwin RL, Lancki DW, Herold KC, Fitch FW. An antigen receptor-driven, interleukin 2-independent pathway for proliferation of murine cytolytic T lymphocyte clones.

Current Protocols in Immunology is a comprehensive source for protocols and overviews in the field of immunology including, in vivo and in vitro assays to study lymphocyte cells, the isolation and differentiation of stem and progenitor cells, flow cytometry and cell sorting, and animal models of infections disease.

Dcnisc M. Hammond‐Mckibben, Aruna Seth, Prakash S. Nagarkatti, Mitzi Nagarkatti, Characterization of factors regulating successful immunotherapy using a tumor‐specific cytotoxic T lymphocyte clone: Role of interleukin‐2, cycling pattern of lytic activity and adhesion molecules, International Journal of Cancer, /ijc,   CYTOLYTIC T-CELL CLONES AND HYBRIDOMAS [30] dine and [I]iododeoxyuridine)°,H To study the release of these nucleo- tides, the supernatants of macrophage cultures have to be tested for their activity to reduce the incorporation of radioactive nucleotides into tumor cells and the test has to be compared with data from the visual cell counting assay.

[30]. Therefore, we cultured and cloned tumorinfiltrating lymphocytes (TIL) from a patient with cervical carcinoma and studied the in vitro characteristics of these TIL by using the established autologous tumor‐cell line.

After stimulation of bulk TIL cultures with 1, Units/ml recombinant interleukin 2 (rIL‐2), followed by limiting dilution, T. Characterization of type I and II clones showed that they are CD8 + and CD4 + CTL clones, respectively, and that the Fas/Fas-L system is involved in their cytotoxicity.

Moreover, the noncytotoxic CD4 + clones, type III, were found to produce and secrete tumor necrosis factor β (TNFβ) 15, 16 after antigen stimulation. Isolation, expansion, and characterization of human natural and adaptive regulatory T cells.

Silvia Gregori, much effort has been placed on developing protocols to generate regulatory T-cell lines and clones, to further define the similarities and differences between various regulatory T-cell subsets. Regulatory T-Lymphocytes.

Figure 1. Typical EasySep™ Direct Human Total Lymphocyte Isolation Profile. Starting with human whole blood from normal healthy donors, the typical total lymphocyte (CD3-CD19+ and CD3+) content of the non-lysed final isolated fraction is ± % (gated on CD45) or ± % (not gated on CD45).

The cloning of T lymphocytes Ronald H. Schwartz Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, MDU.S.A. A new era of celhdar immunology is clearly at hand.

It is now possible, with a little bit of ef/brt, to isolate monoclonal populations of T. Comparing results from immunohistochemistry and flow cytometry, it is estimated that only 20% of T cells can be recovered from human gut biopsies by enzymatic digestion.

25 In this study, we report an improved protocol for the isolation of lymphocytes from human gut biopsies and describe the effect of various types of collagenase on the.

Chronic periodontitis is characterized by dense infiltrations of B and T lymphocytes within the gingival connective tissue. Distinct anaerobic gram-negative bacteria as well as autoimmunity to collagen have been reported to play a role in the etiology and the pathogenesis of this disease.

Here we describe the cloning and characterization of CD4+ and CD8+ T lymphocytes isolated from inflamed. Isolation of viable circulating tumor cells (CTC) holds the promise for improving screening, early diagnosis, and personalized treatment of lymphoma.

In this study, we isolated and characterized spontaneously immortalized B‐lymphocyte (SIBC) lines from HIV‐infected patients with and without Non‐Hodgkin's Lymphoma (AIDS‐NHL).Mehra V, Bloom BR, Torigian VK, Mandich D, Reichel M, Young SM, Salgame P, Convit J, Hunter SW, McNeil M, et al.

Characterization of Mycobacterium leprae cell wall-associated proteins with the use of T lymphocyte clones. J Immunol. Apr 15; (8)– Hunter SW, Rivoire B, Mehra V, Bloom BR, Brennan PJ.Chicory (Cichorium intybus) sesquiterpene lactones were recently shown to be derived from a common sesquiterpene intermediate, (+)-germacrene A.

Germacrene A is of interest because of its key role in sesquiterpene lactone biosynthesis and because it is an enzyme-bound intermediate in the biosynthesis of a number of phytoalexins.

Using polymerase chain reaction with degenerate primers, we.